Scientific research in the Department of natural and mathematical sciences is being carried out in four laboratories. The head of Department is Dr. Dejan Milenković, senior research associate
Laboratory for chemoinformatics and molecular design aims to use computers and information technology to solve problems in the field of chemistry. The combination of information resources enables faster and better decisions in the areas of identification and optimization of known as well as newly synthesized chemical compounds. Also, knowledge of the behavior of chemical processes is necessary for a deeper study of chemistry as a science. Thanks to sophisticated computer programs and resources that can simulate the behavior of molecules and chemical changes, and then visualize the obtained data, they significantly facilitate the understanding of complex chemical processes. The work of researchers in this laboratory who participate in the creation, organization, retrieval, analysis, dissemination and visualization of chemical information aims to apply the results obtained in various industries and design compounds with potential biological and pharmacological properties.
The Laboratory for Bioinorganic and Bioorganic Chemistry aims to synthesize and characterize new pharmacologically important compounds, transition metal complexes and biocompatible ionic liquids. Their biological profiling is primarily performed on a wide range of tumor cell lines by combining modern experimental-computer techniques such as: ADME-Tox, molecular docking, HPLC, emission spectroscopy, flow cytometry, proteomics, MTT and many others. After preliminary in vitro tests, the most active molecules go into the in vivo phase. The mechanism of action, interactions with selected targets (DNA, albumin and specific enzymes), bioavailability as well as packaging methods (nanocarrier and / or ionic liquid) of a potentially active pharmaceutical is an important line of research in this sector. The main goal of these preclinical studies is to find the molecules that have the greatest antitumor potential and that will be used in further phases of the study. It is important to mention that the achieved results within the sector give a significant contribution not only to bio (in) organic chemistry but also to synthetic methodology, in terms of finding new and improved (green) synthetic approaches to new bioactive molecules.
The Laboratory for Bioinformatics and Applied Biology was founded in 2020 at the Institute of Information Technology Kragujevac, University of Kragujevac. The Laboratory has engaged a multidisciplinary team of young researchers with a scientific focus on biomedical and tissue engineering, organ-on-chip technology and biosensors. The main areas of research include, among others, the development of new materials for nanofiber production, drug transport, tissue engineering and regenerative medicine, nanomedicine, the development of domestic organ-on-chip technology and the study of cellular molecular mechanisms at the genetic and protein levels. The group of researchers predominantly comes from the Faculty of Natural Sciences and Mathematics, University of Kragujevac and is one of the most promising scientific groups at the University of Kragujevac. The goal of the research group of the Laboratory is the development of new biomaterials (hydrogels, scaffolds, membranes, nano / microparticles) based on natural polymers for use in drug delivery and tissue engineering. This is achieved by the development of electrospinning methods and 3D bioprinting, which enables the production of integrated biomaterials. On the other hand, the development of domestic organ-on-chip technology is an important tool in the development of science that is based on the functional replacement of in vivo experiments to a significant extent. The integration of the above-mentioned methods with the methods of cell and molecular biology and biosensors opens up possibilities for a modern scientific approach in biomedical research of new drugs, tissue production and molecular diagnostics. Research in the Laboratories is supported by grants from national and European (H2020) funding agencies. In the last two years, the research group of the Laboratory has been one of the most active groups at the University of Kragujevac in terms of scientific work, innovation and cooperation with other research groups from the University of R. Serbia and abroad. This strategy positions the Group as a continuously growing and reputable center for biomaterials research, tissue engineering and regenerative medicine. The research group is built on the basis of an interdisciplinary research approach, where scientific cooperation with other perspective groups is a feature of its working pattern. Great efforts are being made to create long-term partnerships for cooperation with universities in Europe, Asia and North America, which provide opportunities for student and postdoctoral exchange programs and joint research projects. As part of the efforts to overcome the challenges of the COVID-19 pandemic, the Laboratory decided by the Government of the Republic of Serbia. Serbia received a permit and all the necessary resources for the construction and training of a new Laboratory for PCR testing of SARS-CoV-2 clinical samples within the national strategy for the fight against the pandemic. A special Laboratory for PCR testing according to the highest international standards for the manipulation of dangerous viruses has been built.
The laboratory deals with detection testing and the interactions of alpha, beta, and gamma radiation with matter, using numerical simulations, theoretical models, and experimental methods. Within the laboratory development and application of software for calibration and visualization of traces in solid trace detectors, as well as their verification based on comparison with the results obtained by irradiation and chemical etching of the detector. They study are the mechanisms of emanation, exhalation and various measurement techniques of radon and its descendants, using Monte Carlo simulations, analytical models and experiments. Gamma spectrometry and dosimetric measurements are performed in the laboratory concentration of natural and artificial radionuclides in the environment, with examination of correlations with factors influencing their distribution, retention and bioaccumulation. The analysis of the spectrum of gamma spectrometric measurements is performed using the Maestro software package, while statistical processing of measurement results performed using the SPSS and Sigma Plot programs. Research is being done in the laboratory develop biokinetic models that provide the ability to calculate doses, as retrospectively and prospectively, when the body is in different situations exposed to ionizing radiation. The aim is to study the biological effects of radiation on at the cellular level. The rate of elimination of radioisotopes from organs is determined, estimated dose distribution in the target and organs at risk using Monte Carlo simulations. Theoretical models in the field of strong laser fields are also being developed different spatio-temporal structures of the laser pulse on ionization processes. For data entry, statistical analysis, determination of probabilities, numerical calculations, graphical presentation of the obtained results as well as currently creating a top-notch interactive visualization, the Wolfram Matematica program is used. The laboratory is equipped with a gamma spectrometer in lead protection, alpha spectrometer, RAD7 device (Durridge Company Inc.), as well as active devices for measuring X and gamma radiation: Atomtex (AT1125), KOMO-TM and MiniTRACE, and equipment for electrochemical and chemical corrosion detectors.